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Data sheet of PDPK1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Tr |
More info about PDPK1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10309 |
| Product name: | PDPK1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of PDPK1. |
| Gene id: | 5170 |
| Gene name: | PDPK1 |
| Gene alias: | MGC20087|MGC35290|PDK1|PRO0461 |
| Gene description: | 3-phosphoinositide dependent protein kinase-1 |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human PDPK1. |
| Protein accession: | NP_002604 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human PDK-1. |
| Reactivity: | Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using PDPK1 polyclonal antibody (Cat # PAB10309) shows detection of myc-tagged human PDPK1 at 60 KDa in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4°C with the primary antibody diluted 1 : 750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. |
| Applications: | ELISA,IHC,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Identification of tyrosine phosphorylation sites on 3-phosphoinositide-dependent protein kinase-1 and their role in regulating kinase activity.Park J, Hill MM, Hess D, Brazil DP, Hofsteenge J, Hemmings BA. J Biol Chem. 2001 Oct 5;276(40):37459-71. Epub 2001 Jul 31. |