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Data sheet of PCNA polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Clawed frog,Dog,Human,Monkey,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about PCNA polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10307 |
| Product name: | PCNA polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of PCNA. |
| Gene id: | 5111 |
| Gene name: | PCNA |
| Gene alias: | MGC8367 |
| Gene description: | proliferating cell nuclear antigen |
| Immunogen: | A synthetic peptide corresponding to internal region of human PCNA. |
| Protein accession: | NP_872590;P12004 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:8000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human PCNA protein. |
| Reactivity: | Bovine,Chicken,Clawed frog,Dog,Human,Monkey,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using PCNA polyclonal antibody (Cat # PAB10307) shows detection of PCNA protein in HEK293 (Lane 1) and Jurkat (Lane 2) whole cell extracts. Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1 : 1000. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red). |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA.Haracska L, Unk I, Johnson RE, Phillips BB, Hurwitz J, Prakash L, Prakash S. Mol Cell Biol. 2002 Feb;22(3):784-91. |