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Data sheet of PACRG polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about PACRG polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10304 |
| Product name: | PACRG polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of PACRG. |
| Gene id: | 135138 |
| Gene name: | PACRG |
| Gene alias: | FLJ32724|GLUP|HAK005771|PARK2CRG|RP3-495O10.2 |
| Gene description: | PARK2 co-regulated |
| Immunogen: | A synthetic peptide corresponding to amino acids 204-215 of human PACRG. |
| Protein accession: | Q96M98;AAH30642 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human PACRG protein. Expect reactivity with both splice variants of this protein. |
| Reactivity: | Chicken,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using PACRG polyclonal antibody (Cat # PAB10304) shows detection of aband ~ 40 KDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this bandis blocked when the antibody is pre-incubated withthe immunizing peptide (Lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed byLI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Identification of a novel gene linked to parkin via a bi-directional promoter.West AB, Lockhart PJ, O'Farell C, Farrer MJ. J Mol Biol. 2003 Feb 7;326(1):11-9. |