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Data sheet of SPP1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Human,Pig |
| Host species | Rabbit |
| Applications | ELISA,IHC-P,WB-Re |
More info about SPP1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10297 |
| Product name: | SPP1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SPP1. |
| Gene id: | 6696 |
| Gene name: | SPP1 |
| Gene alias: | BNSP|BSPI|ETA-1|MGC110940|OPN |
| Gene description: | secreted phosphoprotein 1 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to human SPP1. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:20000-1:100000) Western Blot (1:500-1:2000) Immunohistochemistry (1:100-1:300) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes the full-length osteopontin protein (which runs at 66 KDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 KDa MMP-cleaved C-fragment is recognized, but not the 40 KDa N-terminal fragment. |
| Reactivity: | Dog,Human,Pig |
| Application image: |  |
| Application image note: | Immunoblotting of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1:1,000 dilution to detect SPP1 by Western blot. In lane 1, reactivity is shown against 250 ng of human SPP1. Lane 2 shows reactivity of MMP-cleaved SPP1. Use a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection. |
| Applications: | ELISA,IHC-P,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells.Denhardt DT, Chambers AF. J Cell Biochem. 1994 Sep;56(1):48-51. |