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Data sheet of Nrf1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about Nrf1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10295 |
| Product name: | Nrf1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant Nrf1. |
| Gene id: | 18181 |
| Gene name: | Nrf1 |
| Gene alias: | C87038|D6Ertd415e |
| Gene description: | nuclear respiratory factor 1 |
| Immunogen: | Recombinant protein corresponding to amino acids 1-534 of mouse Nrf1. |
| Protein accession: | Q9WU00;AAH05410 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000-1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This protein A purified antibody is directed against mouse NRF1. |
| Reactivity: | Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown). Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500. IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is atrademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | c-MYC apoptotic function is mediated by NRF-1 target genes.Morrish F, Giedt C, Hockenbery D. Genes Dev. 2003 Jan 15;17(2):240-55. |