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Data sheet of NOTCH2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human |
| Host species | Rabbit |
| Applications | ELISA,IHC-P,WB-Tr |
More info about NOTCH2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10294 |
| Product name: | NOTCH2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of NOTCH2. |
| Gene id: | 4853 |
| Gene name: | NOTCH2 |
| Gene alias: | AGS2|hN2 |
| Gene description: | Notch homolog 2 (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to amino acids 2396-2409 of human NOTCH2. |
| Protein accession: | Q04721;NP_077719 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:30000-1:90000) Western Blot (1:400-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antiserum is directed against human NOTCH 2. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. |
| Reactivity: | Chimpanzee,Dog,Human |
| Application image: |  |
| Application image note: | Western blot using NOTCH2 polyclonal antibody (Cat # PAB10294) (intra) antibody shows detection of a band at ~110 KDa correspondingto active NOTCH2 protein (arrowhead). Western Blot analysis was performed for NOTCH2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active NOTCH2 (intra cellular NOTCH2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar timepoints. The band at about 110 kD represents active NOTCH2. This band is not seen in the control cell. The intracellular domain of NOTCH2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milkand probed with NOTCH2 polyclonal antibody at a 1 : 400 dilution. ECL was used as visualization method. |
| Applications: | ELISA,IHC-P,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. Leukemia. 2000 Dec;14(12):2095-102. |