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Data sheet of NOTCH2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC-P |
More info about NOTCH2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10293 |
| Product name: | NOTCH2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of NOTCH2. |
| Gene id: | 4853 |
| Gene name: | NOTCH2 |
| Gene alias: | AGS2|hN2 |
| Gene description: | Notch homolog 2 (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to N-terminus the cleaved N intracellular domain (NICD) of human NOTCH2. |
| Protein accession: | Q04721(human);O35516(mouse);Q9QW30(rat);OMIMref:600275 |
| Form: | Liquid |
| Recommend dilutions: | Sandwich ELISA (1:30000-1:90000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antiserum is directed against human NOTCH 2. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunohistochemical staining with NOTCH2 polyclonal antibody (Cat # PAB10293) was diluted 1 : 500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. |
| Applications: | WB,ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. Leukemia. 2000 Dec;14(12):2095-102. |