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Data sheet of NOTCH1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about NOTCH1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10291 |
| Product name: | NOTCH1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of NOTCH1. |
| Gene id: | 4851 |
| Gene name: | NOTCH1 |
| Gene alias: | TAN1|hN1 |
| Gene description: | Notch homolog 1, translocation-associated (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to N-terminus the cleaved N intracellular domain (NICD) of human NOTCH1. |
| Protein accession: | P46531 (human) |
| Form: | Liquid |
| Recommend dilutions: | Sandwich ELISA (1:20000-1:60000) Western Blot (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antiserum is directed against human NOTCH 1. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunoblotting of NOTCH1 polyclonal antibody (Cat # PAB10291) was used at a 1 : 500 dilution to detect mouse NOTCH1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing, reaction with a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development. Lane 1 : No transfection. Lane 2 : N1 (mouse deleted extracellular domain)-myc. Lane 3 : N1 (mouse intracellular domain)-myc. Lane 4 : N2 (mouse deleted extracellular domain)-myc. Lane 5 : N2 (mouse intracellular domain)-myc. Lane 6 : N3 (mouse deleted extracellular domain)-myc. Lane 7 : N3 (mouse intracellular domain)-myc. Lane 8 : N4 (mouse deleted extracellular domain)-myc. Lane 9 : N4 (mouse intracellular domain)-myc. Lane 10 : N1 (mouse deleted extracellular domain) (V to G)-myc. Personal communication, Dr. Stacey Huppert. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW. Leukemia. 2000 Dec;14(12):2095-102. |