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NCOA3 polyclonal antibody

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Data sheet of NCOA3 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Dog,Human,Macaque,Mouse,Rat
Host speciesRabbit
ApplicationsWB,IP,ELISA

More info about NCOA3 polyclonal antibody

Brand: Abnova
Reference: PAB10282
Product name: NCOA3 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of NCOA3.
Gene id: 8202
Gene name: NCOA3
Gene alias: ACTR|AIB-1|AIB1|CAGH16|CTG26|KAT13B|MGC141848|RAC3|SRC3|TNRC14|TNRC16|TRAM-1|bHLHe42|pCIP
Gene description: nuclear receptor coactivator 3
Immunogen: A synthetic peptide corresponding to internal region of human NCOA3.
Protein accession: NP_858045;Q9Y6Q9
Form: Liquid
Recommend dilutions: ELISA (1:175000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f).
Reactivity: Bovine,Dog,Human,Macaque,Mouse,Rat
Application image: PAB10282-12-multi-1.jpg
Application image note: Western blot using NCOA3 polyclonal antibody (Cat # PAB10282) shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4).
The band at ~ 148 kDa, indicated by the arrowhead, corresponds to NCOA3.
Mouse NCOA3 (lanes 1 and 4) appear as ~ 105 kDa bands.
Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown).
Personal communication, H. Ying, NCI, Bethesda, MD.
Applications: WB,IP,ELISA
Shipping condition: Dry Ice
Publications: Steroid receptor coactivator 3 is a coactivator for myocardin, the regulator of smooth muscle transcription and differentiation.Li HJ, Haque Z, Lu Q, Li L, Karas R, Mendelsohn M.
Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):4065-70. Epub 2007 Feb 28.

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