Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of MRE11 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP |
More info about MRE11 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10277 |
| Product name: | MRE11 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MRE11. |
| Gene id: | 855264 |
| Gene name: | MRE11 |
| Gene alias: | NGS1|RAD58|XRS4 |
| Gene description: | Mre11p |
| Immunogen: | A synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae MRE11. |
| Protein accession: | P32829;NP_013951 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Specificity: | This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein. |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Chromatin Immunoprecipitation (ChIP) using MRE11 polyclonal antibody (Cat # PAB10277). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD. |
| Applications: | WB,ELISA,ChIP |
| Shipping condition: | Dry Ice |
| Publications: | Functions of the yeast meiotic recombination genes, MRE11 and MRE2.Ogawa H, Johzuka K, Nakagawa T, Leem SH, Hagihara AH. Adv Biophys. 1995;31:67-76. |