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Data sheet of Rif1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about Rif1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10275 |
| Product name: | Rif1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Rif1. |
| Gene id: | 51869 |
| Gene name: | Rif1 |
| Gene alias: | 5730435J01Rik|6530403D07Rik|AU016181|AW549474|D2Ertd145e |
| Gene description: | Rap1 interacting factor 1 homolog (yeast) |
| Immunogen: | A synthetic peptide corresponding to amino acids 2406-2419 of mouse Rif1. |
| Protein accession: | Q7TPD8;Q6PR54 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:40000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This affinity purified antibody is directed against mouse Rif1 protein. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 KDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 KDa) and mouse Rap1-interacting factor 1 (2426 267.1 KDa). |
| Reactivity: | Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells.Adams IR, McLaren A. Dev Dyn. 2004 Apr;229(4):733-44. |