Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of Mdm2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about Mdm2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10274 |
| Product name: | Mdm2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Mdm2. |
| Gene id: | 17246 |
| Gene name: | Mdm2 |
| Gene alias: | 1700007J15Rik|AA415488|Mdm-2 |
| Gene description: | transformed mouse 3T3 cell double minute 2 |
| Immunogen: | A synthetic peptide corresponding to amino acids 177-195 of mouse Mdm2. |
| Protein accession: | P23804 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000-1:12000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | Reactivity occurs against Mouse MDM2 protein. |
| Reactivity: | Mouse |
| Application image: |  |
| Application image note: | Western blot using Mdm2 polyclonal antibody (Cat # PAB10274) is shown to detect a band (arrow) corresponding to mouse Mdm2 protein present in mouse MEF cells (Lane 2), but not human kidney HEK293 cells (lane1). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at roomtemperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y.Pospisilova S, Siligan C, Ban J, Jug G, Kovar H. Mol Cancer Res. 2004 May;2(5):296-304. |