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Data sheet of Hif3a polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about Hif3a polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10271 |
| Product name: | Hif3a polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Hif3a. |
| Gene id: | 53417 |
| Gene name: | Hif3a |
| Gene alias: | Ipas|MOP7 |
| Gene description: | hypoxia inducible factor 3, alpha subunit |
| Immunogen: | A synthetic peptide corresponding to amino acids 581-592 of mouse Hif3a. |
| Protein accession: | Q9Z2I5;NP_058564 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:14000-1:80000) Western Blot (1:10500-1:8000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This affinity-purified antibody is directed against mouse Hif3alpha protein. No reactivity is expected against other forms of Hif proteins. |
| Reactivity: | Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Hif3a polyclonal antibody (Cat # PAB10271) shows detection of aband ~72 KDa corresponding to mouse Hif3a (arrowhead). Approximately 10 ug of a CoCl2 treated NIH/3T3 cell lysate (Lane 1) and control NIH/3T3 cell lysate (Lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing NIH/3T3 cells with 130 uM CoCl2 for 6 h at 37°C effectively mimicshypoxia. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Cross-talk between the aryl hydrocarbon receptor and hypoxia inducible factor signaling pathways. Demonstration of competition and compensation.Chan WK, Yao G, Gu YZ, Bradfield CA. J Biol Chem. 1999 Apr 23;274(17):12115-23. |