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Gli2 polyclonal antibody

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Data sheet of Gli2 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityMouse,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ti,IHC-P

More info about Gli2 polyclonal antibody

Brand: Abnova
Reference: PAB10270
Product name: Gli2 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Gli2.
Gene id: 14633
Gene name: Gli2
Gene alias: AW546128
Gene description: GLI-Kruppel family member GLI2
Immunogen: A synthetic peptide corresponding to internal region of mouse Gli2.
Protein accession: Q8K0K3(fragment);XP_136212
Form: Liquid
Recommend dilutions: ELISA (1:15000-1:60000)
Western Blot (1:500-1:2000)
Immunohistochemistry (2-20 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Mouse
Specificity: This affinity purified antibody is directed against mouse Gli-2 protein.
Reactivity: Mouse,Rat
Application image: PAB10270-48-69-1.jpg
Application image note: Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
Moderate to strong staining was seen on many tissues with low background staining.
This image shows Gli2 staining of mouse brain.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Applications: ELISA,WB-Ti,IHC-P
Shipping condition: Dry Ice
Publications: Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation.McDermott A, Gustafsson M, Elsam T, Hui CC, Emerson CP Jr, Borycki AG.
Development. 2005 Jan;132(2):345-57. Epub 2004 Dec 16.

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