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Data sheet of MCM2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat,Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about MCM2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10264 |
| Product name: | MCM2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MCM2. |
| Gene id: | 4171 |
| Gene name: | MCM2 |
| Gene alias: | BM28|CCNL1|CDCL1|D3S3194|KIAA0030|MGC10606|MITOTIN|cdc19 |
| Gene description: | minichromosome maintenance complex component 2 |
| Immunogen: | A synthetic peptide corresponding to amino acids 21-31 of human MCM2. |
| Protein accession: | NP_004517;P49736 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity-purified antibody is directed against human MCM2 protein. Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27). |
| Reactivity: | Human,Mouse,Rat,Yeast |
| Application image: |  |
| Application image note: | Western blot using MCM2 polyclonal antibody (Cat # PAB10264) shows detection of both phosphorylated and unphosphorylated MCM2 presentin nuclear extracts from elutriated human cells (MO59K/K-562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the proteinis unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (Lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (Lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. Asynchronous cells contain a doublet of both forms. Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate. The phosphorylated band migrates faster than the unphosphorylated form and isseen as the lower band. There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody. The primary antibody was diluted 1:400 for this experiment. Personal Communication, Jennifer Seiler,NIH CCR Bethesda MD. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.Todorov IT, Pepperkok R, Philipova RN, Kearsey SE, Ansorge W, Werner D. J Cell Sci. 1994 Jan;107 ( Pt 1):253-65. |