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Ldb1 polyclonal antibody

PAB10257_100ug

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549,00 € tax excl.

100 ug

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Data sheet of Ldb1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityChicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ce

More info about Ldb1 polyclonal antibody

Brand: Abnova
Reference: PAB10257
Product name: Ldb1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Ldb1.
Gene id: 16825
Gene name: Ldb1
Gene alias: CLIM2|NLI
Gene description: LIM domain binding 1
Immunogen: A synthetic peptide corresponding to amino acids 361-373 of mouse Ldb1.
Protein accession: P70662;NP_034827
Form: Liquid
Recommend dilutions: ELISA (1:170000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Mouse
Specificity: This affinity purified antibody is directed against the mouse LDB1protein.
Reactivity: Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat
Application image: PAB10257-46-6-1.jpg
Application image note: Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate.
Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose.
Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1:20,000 dilution of DyLightâ„¢800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Applications: ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: Genomic structure and chromosomal localization of the mouse LIM domain-binding protein 1 gene, Ldb1.Yamashita T, Agulnick AD, Copeland NG, Gilbert DJ, Jenkins NA, Westphal H.
Genomics. 1998 Feb 15;48(1):87-92.

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