Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
Host species | Rabbit |
Applications | ELISA,WB-Ce |
Brand: | Abnova |
Reference: | PAB10257 |
Product name: | Ldb1 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Ldb1. |
Gene id: | 16825 |
Gene name: | Ldb1 |
Gene alias: | CLIM2|NLI |
Gene description: | LIM domain binding 1 |
Immunogen: | A synthetic peptide corresponding to amino acids 361-373 of mouse Ldb1. |
Protein accession: | P70662;NP_034827 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:170000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Mouse |
Specificity: | This affinity purified antibody is directed against the mouse LDB1protein. |
Reactivity: | Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Rat |
Application image: | ![]() |
Application image note: | Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
Applications: | ELISA,WB-Ce |
Shipping condition: | Dry Ice |
Publications: | Genomic structure and chromosomal localization of the mouse LIM domain-binding protein 1 gene, Ldb1.Yamashita T, Agulnick AD, Copeland NG, Gilbert DJ, Jenkins NA, Westphal H. Genomics. 1998 Feb 15;48(1):87-92. |