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Data sheet of lacI polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about lacI polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10255 |
| Product name: | lacI polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant lacI. |
| Gene id: | 945007 |
| Gene name: | lacI |
| Gene alias: | ECK0342|JW0336 |
| Gene description: | DNA-binding transcriptional repressor |
| Immunogen: | Recombinant protein corresponding to full length Escherichia coli (strain DH10B) lacI. |
| Protein accession: | AAA24052;P03023 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:90000-1:100000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Escherichia coli |
| Specificity: | This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. |
| Application image: |  |
| Application image note: | Western blot using lacI polyclonal antibody (Cat # PAB10255) shows detection of a 38 kDa band corresponding to recombinant lacI (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1 : 1,000 dilution followed by reaction with a 1 : 10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium.Camsund D, Heidorn T, Lindblad P J Biol Eng. 2014 Jan 27;8(1):4. doi: 10.1186/1754-1611-8-4. |