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Data sheet of WRNIP1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Monkey,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC,WB-Ce |
More info about WRNIP1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10242 |
| Product name: | WRNIP1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of WRNIP1. |
| Gene id: | 56897 |
| Gene name: | WRNIP1 |
| Gene alias: | FLJ22526|RP11-420G6.2|WHIP|bA420G6.2 |
| Gene description: | Werner helicase interacting protein 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human WRNIP1. |
| Protein accession: | Q96S55;CAH73664 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 KDa and 69.5 KDa, respectively. |
| Reactivity: | Human,Monkey,Mouse,Rat |
| Application image: |  |
| Application image note: | Fig. 2 Western blot analysis is shown using WRNIP1 polyclonal antibody (Cat # PAB10242) with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WRNIP1. Approximately 30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 KDa is detected. The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min. |
| Applications: | WB,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | A novel protein interacts with the Werner's syndrome gene product physically and functionally.Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T. J Biol Chem. 2001 Jun 8;276(23):20364-9. Epub 2001 Apr 11. |