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USP18 polyclonal antibody

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Data sheet of USP18 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityChimpanzee,Dog,Human,Mouse,Orangutan
Host speciesRabbit
ApplicationsELISA,WB-Ce,IF

More info about USP18 polyclonal antibody

Brand: Abnova
Reference: PAB10241
Product name: USP18 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of USP18.
Gene id: 11274
Gene name: USP18
Gene alias: ISG43|UBP43
Gene description: ubiquitin specific peptidase 18
Immunogen: A synthetic peptide corresponding to amino acids 40-49 of human USP18.
Protein accession: Q9UMW8;NP_059110
Form: Liquid
Recommend dilutions: ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This affinity-purified antibody is directed against human UBP43 protein.
Reactivity: Chimpanzee,Dog,Human,Mouse,Orangutan
Application image: PAB10241-46-multi-1.jpg
Application image note: Western blot using USP18 polyclonal antibody (Cat # PAB10241) shows detection of a band ~43 KDa band (arrowhead) believed to be to USP18 in lysates from HeLa nuclear extracts (Lane 1) and whole cell lysates from HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and HEK293 (Lane 5).
Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.
Signal was detected using standard techniques.
The strong band between the 37 KDa and 50 KDa markers is expected to be USP18.
The other bands are probably non-specific.
Applications: ELISA,WB-Ce,IF
Shipping condition: Dry Ice
Publications: RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response.Li XL, Blackford JA, Judge CS, Liu M, Xiao W, Kalvakolanu DV, Hassel BA.
J Biol Chem. 2000 Mar 24;275(12):8880-8.

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