Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of TLK1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Human,Mouse,Orangutan,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about TLK1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10238 |
| Product name: | TLK1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of TLK1. |
| Gene id: | 9874 |
| Gene name: | TLK1 |
| Gene alias: | KIAA0137|PKU-beta |
| Gene description: | tousled-like kinase 1 |
| Immunogen: | A synthetic peptide corresponding to amino acids 737-751 of human TLK1. |
| Protein accession: | Q9UKI8;NP_036422(isoform1) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. This antibody will react with all isoforms of Tlk-1. |
| Reactivity: | Chicken,Chimpanzee,Dog,Human,Mouse,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using TLK1 polyclonal antibody (Cat # PAB10238) shows detection of aband ~ 64 KDa corresponding to isoform 3 of human TLK1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 250 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeâ„¢800 conjugated Gt-a-RabbitIgG [H&L] MX for 45 min at room temperature. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.Groth A, Lukas J, Nigg EA, Sillje HH, Wernstedt C, Bartek J, Hansen K. EMBO J. 2003 Apr 1;22(7):1676-87. |