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RNF2 polyclonal antibody

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Data sheet of RNF2 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Orangutan,Rat
Host speciesGoat
ApplicationsWB,ELISA

More info about RNF2 polyclonal antibody

Brand: Abnova
Reference: PAB10232
Product name: RNF2 polyclonal antibody
Product description: goat polyclonal antibody raised against synthetic peptide of RNF2.
Gene id: 6045
Gene name: RNF2
Gene alias: BAP-1|BAP1|DING|HIPI3|RING1B|RING2
Gene description: ring finger protein 2
Immunogen: A synthetic peptide corresponding to amino acids 189-201 of human RNF2.
Protein accession: Q99496;NP_009143
Form: Liquid
Recommend dilutions: ELISA (1:5000-1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Goat
Antigen species / target species: Human
Specificity: This affinity purified antibody is directed against human RING1B protein.
Reactivity: Bovine,Chicken,Chimpanzee,Dog,Frog,Human,Mouse,Orangutan,Rat
Application image: PAB10232-12-multi-1.jpg
Application image note: Western blot using RNF2 polyclonal antibody (Cat # PAB10232) shows detection of a 38 KDa band corresponding to human RNF2 in 3T3 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), mouse brain (Lane 4) and CHO-K1 (Lane 5) cell lysates.
Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilutionof RNF2 polyclonal antibody incubated at room temperature.
Signal was detected using standard techniques.
Applications: WB,ELISA
Shipping condition: Dry Ice
Publications: Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.Tuckfield A, Clouston DR, Wilanowski TM, Zhao LL, Cunningham JM, Jane SM.
Mol Cell Biol. 2002 Mar;22(6):1936-46.

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