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Data sheet of RAD52 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about RAD52 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10230 |
| Product name: | RAD52 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of RAD52. |
| Gene id: | 5893 |
| Gene name: | RAD52 |
| Gene alias: | - |
| Gene description: | RAD52 homolog (S. cerevisiae) |
| Immunogen: | A synthetic peptide corresponding to amino acids 360-375 of human RAD52. |
| Protein accession: | P43351;NP_002870 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human RAD52 protein. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot analysis is shown using RAD52 polyclonal antibody (Cat # PAB10230) to detect human RAD52 present in a HeLa nuclear extract (panel B). Approximately 30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~ 63.0 KDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1 : 1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDyeâ„¢800 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 5,000 for 45 min. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues.Shen Z, Denison K, Lobb R, Gatewood JM, Chen DJ. Genomics. 1995 Jan 1;25(1):199-206. |