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Data sheet of NIPBL polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Human,Mouse,Orangutan,Rat |
| Host species | Goat |
| Applications | ELISA,WB-Ti |
More info about NIPBL polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10226 |
| Product name: | NIPBL polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of NIPBL. |
| Gene id: | 25836 |
| Gene name: | NIPBL |
| Gene alias: | CDLS|CDLS1|DKFZp434L1319|FLJ11203|FLJ12597|FLJ13354|FLJ13648|FLJ44854|IDN3|IDN3-B |
| Gene description: | Nipped-B homolog (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to amino acids 344-356 of human NIPBL. |
| Protein accession: | Q6KC79;BAA77335 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human IDN3 protein. |
| Reactivity: | Bovine,Dog,Human,Mouse,Orangutan,Rat |
| Application image: |  |
| Application image note: | Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of a band ~300 KDa correspondingto NIPBL (arrowhead) in human kidney (lane1) and human heart (Lane 2) whole cell tissue extracts. Approximately 20 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome.Tonkin ET, Wang TJ, Lisgo S, Bamshad MJ, Strachan T. Nat Genet. 2004 Jun;36(6):636-41. Epub 2004 May 16. |