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USP7 polyclonal antibody

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Data sheet of USP7 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ce

More info about USP7 polyclonal antibody

Brand: Abnova
Reference: PAB10225
Product name: USP7 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of USP7.
Gene id: 7874
Gene name: USP7
Gene alias: HAUSP|TEF1
Gene description: ubiquitin specific peptidase 7 (herpes virus-associated)
Immunogen: A synthetic peptide corresponding to N-terminus of human USP7.
Protein accession: NP_003461;Q93009
Form: Liquid
Recommend dilutions: ELISA (1:15000-1:60000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat
Application image: PAB10225-46-multi-1.jpg
Application image note: Western blot using USP7 polyclonal antibody (Cat # PAB10225) shows detection of USP7 in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A-431, Lane 4 - MCF-7, Lane 5 - 3T3).
The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.
A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.
Exposure time was 30 sec.
Applications: ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: Expression and functional analyses of mHAUSP regulating apoptosis of cervical adenocarcinoma cells.Yoo KJ, Lee HJ, Lee H, Lee KY, Lee SH, Chung HM, Baek KH.
Int J Oncol. 2005 Jul;27(1):97-104.

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