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Data sheet of ATAD5 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about ATAD5 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10222 |
| Product name: | ATAD5 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ATAD5. |
| Gene id: | 79915 |
| Gene name: | ATAD5 |
| Gene alias: | C17orf41|FLJ12735|FRAG1 |
| Gene description: | ATPase family, AAA domain containing 5 |
| Immunogen: | A synthetic peptide corresponding to amino acids 63-76 of human ATAD5. |
| Protein accession: | Q96QE3;CAC44537 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000-1:15000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen. |
| Reactivity: | Chimpanzee,Dog,Human |
| Application image: |  |
| Application image note: | Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates. Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time. The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa. The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown). |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions.Jenne DE, Tinschert S, Stegmann E, Reimann H, Nurnberg P, Horn D, Naumann I, Buske A, Thiel G. Genomics. 2000 May 15;66(1):93-7. |