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Data sheet of AP1G1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human,Orangutan |
| Host species | Goat |
| Applications | IP,ELISA,WB-Ce |
More info about AP1G1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10221 |
| Product name: | AP1G1 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of AP1G1. |
| Gene id: | 164 |
| Gene name: | AP1G1 |
| Gene alias: | ADTG|CLAPG1|MGC18255 |
| Gene description: | adaptor-related protein complex 1, gamma 1 subunit |
| Immunogen: | A synthetic peptide corresponding to amino acids 646-659 of human AP1G1. |
| Protein accession: | O43747;NP_001119 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:8000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Human,Orangutan |
| Application image: |  |
| Application image note: | Western blot using AP1G1 polyclonal antibody (Cat # PAB10221) shows strong detection of a 91 KDa band corresponding to human AP1G1 in a HeLa whole cell lysate (Lane 1). Peptide competition (using 1 ug/mL of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (Lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of AP1G1 polyclonal antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 60-sec exposure time. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Identification and characterization of novel clathrin adaptor-related proteins.Takatsu H, Sakurai M, Shin HW, Murakami K, Nakayama K. J Biol Chem. 1998 Sep 18;273(38):24693-700. |