Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Chicken,Fruit fly,Human,Monkey,Mouse,Rat |
Host species | Rabbit |
Applications | ELISA,WB-Ce,IHC-P |
Brand: | Abnova |
Reference: | PAB10219 |
Product name: | HSP90AA1 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1. |
Gene id: | 3320 |
Gene name: | HSP90AA1 |
Gene alias: | FLJ31884|HSP86|HSP89A|HSP90A|HSP90N|HSPC1|HSPCA|HSPCAL1|HSPCAL4|HSPN|Hsp89|Hsp90|LAP2 |
Gene description: | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
Immunogen: | A synthetic peptide corresponding to residues surrounding K294 of human HSP90AA1. |
Protein accession: | P46633;P07900 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:2500-1:10000) Western Blot (1:500-1:2500) Immunohistochemistry (5-10 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Specificity: | This affinity purified antibody is directed against human Hsp90 protein acetylated at K294. |
Reactivity: | Chicken,Fruit fly,Human,Monkey,Mouse,Rat |
Application image: | ![]() |
Application image note: | Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10219) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in an SK-BR-3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). Immuno-precipitation with HSP90AA1 polyclonal antibody was performed prior to immunoblotting with anti-HSP90AA1 acetyl K294. |
Applications: | ELISA,WB-Ce,IHC-P |
Shipping condition: | Dry Ice |
Publications: | Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K. Mol Cancer Ther. 2003 Oct;2(10):971-84. |