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HSP90AA1 polyclonal antibody

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Data sheet of HSP90AA1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityChicken,Fruit fly,Human,Monkey,Mouse,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ce,IHC-P

More info about HSP90AA1 polyclonal antibody

Brand: Abnova
Reference: PAB10219
Product name: HSP90AA1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1.
Gene id: 3320
Gene name: HSP90AA1
Gene alias: FLJ31884|HSP86|HSP89A|HSP90A|HSP90N|HSPC1|HSPCA|HSPCAL1|HSPCAL4|HSPN|Hsp89|Hsp90|LAP2
Gene description: heat shock protein 90kDa alpha (cytosolic), class A member 1
Immunogen: A synthetic peptide corresponding to residues surrounding K294 of human HSP90AA1.
Protein accession: P46633;P07900
Form: Liquid
Recommend dilutions: ELISA (1:2500-1:10000)
Western Blot (1:500-1:2500)
Immunohistochemistry (5-10 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This affinity purified antibody is directed against human Hsp90 protein acetylated at K294.
Reactivity: Chicken,Fruit fly,Human,Monkey,Mouse,Rat
Application image: PAB10219-46-10-1.jpg
Application image note: Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10219) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in an SK-BR-3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor).
Western blotting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA).
Immuno-precipitation with HSP90AA1 polyclonal antibody was performed prior to immunoblotting with anti-HSP90AA1 acetyl K294.
Applications: ELISA,WB-Ce,IHC-P
Shipping condition: Dry Ice
Publications: Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K.
Mol Cancer Ther. 2003 Oct;2(10):971-84.

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