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Data sheet of HSP90AA1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Fruit fly,Human,Monkey,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Tr |
More info about HSP90AA1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10218 |
| Product name: | HSP90AA1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of HSP90AA1. |
| Gene id: | 3320 |
| Gene name: | HSP90AA1 |
| Gene alias: | FLJ31884|HSP86|HSP89A|HSP90A|HSP90N|HSPC1|HSPCA|HSPCAL1|HSPCAL4|HSPN|Hsp89|Hsp90|LAP2 |
| Gene description: | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Immunogen: | A synthetic peptide corresponding to amino acids 289-300 of human HSP90AA1. |
| Protein accession: | P46633;P07900 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Fruit fly,Human,Monkey,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using HSP90AA1 polyclonal antibody (Cat # PAB10218) shows detection of a band at ~90 KDa corresponding to HSP90AA1 in various lysate preparations (arrowhead). COS-7 transfected with mutant K294R HSP90AA1 (left two lanes), COS-7 transfected with wild type HSP90AA1 (middle two lanes) and empty vector CSO-7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only, as indicated. Transfected cells express either mutant or wt HSP90AA1 coupled to Flagâ„¢tag. Anti-Flagâ„¢ immunoprecipitation was performed prior to immunoblotting with HSP90AA1 polyclonal antibody. Personal Communication, Brad Scroggins, Urologic Oncology Branch, CCR, NCI, Rockville, MD. |
| Applications: | ELISA,IHC,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Histone deacetylase inhibitor LAQ824 down-regulates Her-2 and sensitizes human breast cancer cells to trastuzumab, taxotere, gemcitabine, and epothilone B.Fuino L, Bali P, Wittmann S, Donapaty S, Guo F, Yamaguchi H, Wang HG, Atadja P, Bhalla K. Mol Cancer Ther. 2003 Oct;2(10):971-84. |