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Data sheet of Fap polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about Fap polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10209 |
| Product name: | Fap polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Fap. |
| Gene id: | 14089 |
| Gene name: | Fap |
| Gene alias: | - |
| Gene description: | fibroblast activation protein |
| Immunogen: | A synthetic peptide corresponding to internal region of mouse Fap. |
| Protein accession: | NP_032012;P97321 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This antibody is known to react with mouse FAP protein. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Fap polyclonal antibody (Cat # PAB10209) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells (Lane 4), but not control HEK cells (Lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000. Personal communication, S.Kim, NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Expression of the fibroblast activation protein during mouse embryo development.Niedermeyer J, Garin-Chesa P, Kriz M, Hilberg F, Mueller E, Bamberger U, Rettig WJ, Schnapp A. Int J Dev Biol. 2001 Apr;45(2):445-7. |