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Data sheet of FBXO43 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Tr |
More info about FBXO43 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10206 |
| Product name: | FBXO43 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of FBXO43. |
| Gene id: | 286151 |
| Gene name: | FBXO43 |
| Gene alias: | EMI2|ERP1|Fbx43 |
| Gene description: | F-box protein 43 |
| Immunogen: | A synthetic peptide corresponding to N-terminus 60 amino acids of human FBXO43. |
| Protein accession: | NP_001025031;Q4G163 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity-purified antibody is directed against human Fbp5B protein. |
| Reactivity: | Chimpanzee,Human |
| Application image: |  |
| Application image note: | Western blot using FBXO43 polyclonal antibody (Cat # PAB10206). Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate. Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute. |
| Applications: | IP,ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Systematic analysis and nomenclature of mammalian F-box proteins.Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW. Genes Dev. 2004 Nov 1;18(21):2573-80. |