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Data sheet of Collagen Type I polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Human |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC-P,WB-Tr |
More info about Collagen Type I polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10190 |
| Product name: | Collagen Type I polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against native Collagen Type I. |
| Immunogen: | Native purified human and bovine placenta Collagen Type I. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:50000) Western Blot (1:5000-1:50000) Immunohistochemistry (1:50-1:200) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 100 mM Na2B4O7, 75 mM NaCl, 5 mM EDTA, pH 8.0 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C on dry atmosphere. After dilution, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Native Purified Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Bovine |
| Specificity: | This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Reactivity: | Bovine,Human |
| Application image: |  |
| Application image note: | Western blot analysis is shown using Collagen Type I polyclonal antibody (Cat # PAB10190) to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARgamma-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with Collagen Type I polyclonal antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% Non-fat milk. Detection occurred by incubation with a horseradish peroxidase-conjugated secondary antibody at 1 ug/10 ml. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences). See Hazra et al. (2004) for additional details. |
| Applications: | IP,ELISA,IHC-P,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells.Hazra S, Xiong S, Wang J, Rippe RA, Krishna V, Chatterjee K, Tsukamoto H. J Biol Chem. 2004 Mar 19;279(12):11392-401. Epub 2003 Dec 31. |