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Data sheet of Myb polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Gorilla,Horse,Human,Monkey,Mouse,Opossum,Orangutan,Pig,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about Myb polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10189 |
| Product name: | Myb polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Myb. |
| Gene id: | 17863 |
| Gene name: | Myb |
| Gene alias: | AI550390|M16449|MGC18531|c-myb |
| Gene description: | myeloblastosis oncogene |
| Immunogen: | A synthetic peptide corresponding to internal region of mouse Myb. |
| Protein accession: | NP_034978;P06876 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:25000) Western Blot (1:250-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Reactivity: | Chimpanzee,Gorilla,Horse,Human,Monkey,Mouse,Opossum,Orangutan,Pig,Rat |
| Application image: |  |
| Application image note: | Western blot using Myb polyclonal antibody (Cat # PAB10189) in COS-7 cell lysates. Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce Myb modification. Lane 3 shows detection of bands at 75, 98, and 125 KDa corresponding to overexpressed Myb (arrows) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45 um membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Personal communication, J. Bies, NCI NIH, Bethesda. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.Bies J, Markus J, Wolff L. J Biol Chem. 2002 Mar 15;277(11):8999-9009. Epub 2002 Jan 4. |