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Data sheet of Cd97 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,IF,WB-Tr |
More info about Cd97 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10186 |
| Product name: | Cd97 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant Cd97. |
| Gene id: | 26364 |
| Gene name: | Cd97 |
| Gene alias: | AA409984|TM7LN1 |
| Gene description: | CD97 antigen |
| Immunogen: | Recombinant protein corresponding to amino acids 1-512 of mouse Cd97. |
| Protein accession: | NP_036055;Q9Z0M6 |
| Form: | Lyophilized |
| Recommend dilutions: | Western Blot (1:1000-1:5000) Immunofluorescence (1:1000-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Reactivity: | Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Cd97 polyclonal antibody (Cat # PAB10186) shows detection of bands corresponding to free Fc-Cd97- (5EGF) (lower arrowhead) and Fc-Cd97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-Cd97- (5EGF). No staining was noted from bone marrow lysates taken from Cd97 knockout mice. The identity of the band at ~65 KDa appearing in all lanes is not known. The formation of the Cd97 complex is currently under investigation. Approximately 10 uL of lysate was used in each lane. A 1:1,000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD. |
| Applications: | ELISA,IF,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | CD97, but not its closely related EGF-TM7 family member EMR2, is expressed on gastric, pancreatic, and esophageal carcinomas.Aust G, Steinert M, Schutz A, Boltze C, Wahlbuhl M, Hamann J, Wobus M. Am J Clin Pathol. 2002 Nov;118(5):699-707. |