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Data sheet of Asap1 (phospho Y782) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC-P,IF |
More info about Asap1 (phospho Y782) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10087 |
| Product name: | Asap1 (phospho Y782) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Asap1. |
| Gene id: | 13196 |
| Gene name: | Asap1 |
| Gene alias: | AV239055|Ddef1|PAP|mKIAA1249|s19 |
| Gene description: | ArfGAP with SH# domain, ankyrin repeat and PH domain1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y782 of mouse Asap1. |
| Protein accession: | NP_034156(seealsoAAC98349);Q9QWY8 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:16000) Western Blot (1:500-1:2000) Immunohistochemistry (20-40 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | Reactivity occurs against mouse ASAP1 pY782 protein and This antibody is specific to the phosphorylated form of the protein. |
| Reactivity: | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunofluorescent microscopy using Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) shows detection of phosphorylated Asap1 present in mouse NIH/3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD. |
| Applications: | WB,ELISA,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.Liu Y, Loijens JC, Martin KH, Karginov AV, Parsons JT. Mol Biol Cell. 2002 Jun;13(6):2147-56. |