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Data sheet of ING5 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Frog,Human,Mouse,Rat |
| Host species | Goat |
| Applications | ELISA,WB-Re,WB-Tr |
More info about ING5 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10079 |
| Product name: | ING5 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of ING5. |
| Gene id: | 84289 |
| Gene name: | ING5 |
| Gene alias: | FLJ23842|p28ING5 |
| Gene description: | inhibitor of growth family, member 5 |
| Immunogen: | A synthetic peptide corresponding to amino acids 127-140 of human ING5. |
| Protein accession: | Q8WYH8;NP_115705(isoform1);AAH05370(isoform2) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:16000) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Frog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot analysis is shown using ING5 polyclonal antibody (Cat # PAB10079) to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4on the same membrane (lane 2). A mock purification is shown inlane 1. Comparison to a molecular weight marker (not shown)indicates a single band of ~45.0 KDa corresponding to theexpected molecular weight for the recombinant protein. The blotwas incubated with a 1:500 dilution of the antibody at roomtemperature followed by detection using chemiluminescencereagent with a 5-min exposure time. Other detection systems willyield similar results. Personal communication Jacques Cote. |
| Applications: | ELISA,WB-Re,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.Shiseki M, Nagashima M, Pedeux RM, Kitahama-Shiseki M, Miura K, Okamura S, Onogi H, Higashimoto Y, Appella E, Yokota J, Harris CC. Cancer Res. 2003 May 15;63(10):2373-8. |