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Data sheet of MAD2L1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about MAD2L1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10076 |
| Product name: | MAD2L1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MAD2L1. |
| Gene id: | 4085 |
| Gene name: | MAD2L1 |
| Gene alias: | HSMAD2|MAD2 |
| Gene description: | MAD2 mitotic arrest deficient-like 1 (yeast) |
| Immunogen: | A synthetic peptide corresponding to amino acids 3-13 of human MAD2L1. |
| Protein accession: | Q13257;NP_002349 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using MAD2L1 polyclonal antibody (Cat # PAB10076) shows detection of a predominant band at ~24 KDa corresponding to MAD2L1 (arrowhead) present in Jurkat (Lane 1) and HeLa (Lane 2) whole cell lysates using the 800 nmchannel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide (lanes 4 and 5 respectively) which completely blocks antibody staining. Approximately 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (Lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | Mad2 and p27 expression profiles in colorectal cancer and its clinical significance.Li GQ, Zhang HF. World J Gastroenterol. 2004 Nov 1;10(21):3218-20. |