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Data sheet of PARK7 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Dog,Frog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about PARK7 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10070 |
| Product name: | PARK7 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of PARK7. |
| Gene id: | 11315 |
| Gene name: | PARK7 |
| Gene alias: | DJ-1|DJ1|FLJ27376|FLJ34360|FLJ92274 |
| Gene description: | Parkinson disease (autosomal recessive, early onset) 7 |
| Immunogen: | A synthetic peptide corresponding to amino acids 177-189 of human PARK7. |
| Protein accession: | Q99497;NP_009193 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:2000) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chicken,Dog,Frog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot analysis is shown using PARK7 polyclonal antibody (Cat # PAB10070) to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 KDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,300 dilution ofthe antibody overnight at 4°C followed by detectionusing IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT. IRDye™800fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDyeis a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism.Bonifati V, Rizzu P, van Baren MJ, Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M, van Dongen JW, Vanacore N, van Swieten JC, Brice A, Meco G, van Duijn CM, Oostra BA, Heutink P. Science. 2003 Jan 10;299(5604):256-9. Epub 2002 Nov 21. |