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Data sheet of PLK1 (phospho T210) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Clawed frog,Dog,Flies,Human,Mouse,Pig,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about PLK1 (phospho T210) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10069 |
| Product name: | PLK1 (phospho T210) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PLK1. |
| Gene id: | 5347 |
| Gene name: | PLK1 |
| Gene alias: | PLK|STPK13 |
| Gene description: | polo-like kinase 1 (Drosophila) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T210 of human PLK1. |
| Protein accession: | P53350;NP_005021 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000-1:12000) Western Blot (1:200-1:2000) Immunohistochemistry (1:200-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human Plk-1 protein at the pT210 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Reactivity: | Chicken,Chimpanzee,Clawed frog,Dog,Flies,Human,Mouse,Pig,Rat |
| Application image: |  |
| Application image note: | Immunohistochemistry of PLK1 (phospho T210) polyclonal antibody (Cat # PAB10069) was used at a 1 : 200 dilution to detect PLK1 by immunohistochemistry in human breast carcinoma tissue. Tissue was formalin-fixed and paraffinembedded. Personal Communication, Alan Yen,Life Span Biosciences, Seattle, WA. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH. J Biol Chem. 2004 Aug 27;279(35):36841-54. Epub 2004 Jun 21. |