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SMARCAL1 polyclonal antibody

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Data sheet of SMARCAL1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,WB-Ce

More info about SMARCAL1 polyclonal antibody

Brand: Abnova
Reference: PAB10063
Product name: SMARCAL1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of SMARCAL1.
Gene id: 50485
Gene name: SMARCAL1
Gene alias: HARP|HHARP
Gene description: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
Immunogen: A synthetic peptide corresponding to amino acids 80-96 of human SMARCAL1.
Protein accession: Q9NZC9;NP_003003
Form: Liquid
Recommend dilutions: ELISA (1:16000-1:64000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB10063-46-multi-1.jpg
Application image note: Western blot using SMARCAL1 polyclonal antibody (Cat # PAB10063) shows detection of a band ~106 KDa band (arrowhead) corresponding to SMARCAL1 in human derived cultured cell lysates (HeLa nuclear extract at lane 1, HeLa at lane 2, A-431 at lane 3, Jurkat at lane 4, and 293 at lane 5).
Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of SMARCAL1 polyclonal antibody.
Signal was detected using standard techniques.
SMARCAL1 is the band seen between the 97 and 116kD markers.
Applications: ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse.Coleman MA, Eisen JA, Mohrenweiser HW.
Genomics. 2000 May 1;65(3):274-82.

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