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Data sheet of RAD23B polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Dog,Human,Mouse,Rat,Yeast |
| Host species | Goat |
| Applications | ELISA,WB-Ce |
More info about RAD23B polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10058 |
| Product name: | RAD23B polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of RAD23B. |
| Gene id: | 5887 |
| Gene name: | RAD23B |
| Gene alias: | HHR23B|HR23B|P58 |
| Gene description: | RAD23 homolog B (S. cerevisiae) |
| Immunogen: | A synthetic peptide corresponding to amino acids 163-176 of human RAD23B. |
| Protein accession: | P54727;NP_002865 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:16000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Dog,Human,Mouse,Rat,Yeast |
| Application image: |  |
| Application image note: | Western blot using RAD23B polyclonal antibody (Cat # PAB10058) shows detection of a band at ~58 KDa (arrowhead) corresponding to RAD23B present in a HeLa whole cell lysate. Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data notshown). Approximately 33 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nmchannel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Involvement of the DNA repair protein hHR23 in p53 degradation.Glockzin S, Ogi FX, Hengstermann A, Scheffner M, Blattner C. Mol Cell Biol. 2003 Dec;23(24):8960-9. |