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GLI3 polyclonal antibody

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Data sheet of GLI3 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ti,IHC-P

More info about GLI3 polyclonal antibody

Brand: Abnova
Reference: PAB10052
Product name: GLI3 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of GLI3.
Gene id: 2737
Gene name: GLI3
Gene alias: ACLS|GCPS|PAP-A|PAPA|PAPA1|PAPB|PHS|PPDIV
Gene description: GLI-Kruppel family member GLI3
Immunogen: A synthetic peptide corresponding to amino acids 41-57 of human GLI3.
Protein accession: P10071;NP_000159
Form: Liquid
Recommend dilutions: ELISA (1:6000-1:30000)Western Blot (1:500-1:2000)
Immunohistochemistry (0.5-5 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse,Rat
Application image: PAB10052-48-68-1.jpg
Application image note: Immunohistochemistry of GLI3 polyclonal antibody (Cat # PAB10052) was used at a 0.625 ug/mL to detect GLI3 in a variety of tissues.
Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports.
Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.
This image shows GLI3 staining of human glioblastoma.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Applications: ELISA,WB-Ti,IHC-P
Shipping condition: Dry Ice
Publications: Clinical and molecular delineation of the Greig cephalopolysyndactyly contiguous gene deletion syndrome and its distinction from acrocallosal syndrome.Johnston JJ, Olivos-Glander I, Turner J, Aleck K, Bird LM, Mehta L, Schimke RN, Heilstedt H, Spence JE, Blancato J, Biesecker LG.
Am J Med Genet A. 2003 Dec 15;123A(3):236-42.

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