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Data sheet of GGA3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Macaque,Orangutan |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Tr |
More info about GGA3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10050 |
| Product name: | GGA3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of GGA3. |
| Gene id: | 23163 |
| Gene name: | GGA3 |
| Gene alias: | KIAA0154 |
| Gene description: | golgi associated, gamma adaptin ear containing, ARF binding protein 3 |
| Immunogen: | A synthetic peptide corresponding to amino acids 400-415 of human GGA3. |
| Protein accession: | Q9NZ52;NP_619525 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein. |
| Reactivity: | Human,Macaque,Orangutan |
| Application image: |  |
| Application image note: | Western blot using GGA3 polyclonal antibody (Cat # PAB10050) shows detection of aband at ~110 KDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (Lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane3). MW markers are shown in lane 1 (700 nm channel, red). Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:600. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | IP,ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains.Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH. Nature. 2002 Feb 21;415(6874):933-7. |