Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | ELISA,IHC,WB-Ce |
Brand: | Abnova |
Reference: | PAB10044 |
Product name: | SETDB1 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SETDB1. |
Gene id: | 9869 |
Gene name: | SETDB1 |
Gene alias: | ESET|KG1T|KIAA0067|KMT1E |
Gene description: | SET domain, bifurcated 1 |
Immunogen: | A synthetic peptide corresponding to amino acids 1058-1075 of human SETDB1. |
Protein accession: | NP_036564;Q15047 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:1000-1:2500) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate. Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDyeâ¢800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min. IRDyeâ¢800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
Applications: | ELISA,IHC,WB-Ce |
Shipping condition: | Dry Ice |
Publications: | Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids.Harte PJ, Wu W, Carrasquillo MM, Matera AG. Cytogenet Cell Genet. 1999;84(1-2):83-6. |