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SETDB1 polyclonal antibody

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Data sheet of SETDB1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman
Host speciesRabbit
ApplicationsELISA,IHC,WB-Ce

More info about SETDB1 polyclonal antibody

Brand: Abnova
Reference: PAB10044
Product name: SETDB1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of SETDB1.
Gene id: 9869
Gene name: SETDB1
Gene alias: ESET|KG1T|KIAA0067|KMT1E
Gene description: SET domain, bifurcated 1
Immunogen: A synthetic peptide corresponding to amino acids 1058-1075 of human SETDB1.
Protein accession: NP_036564;Q15047
Form: Liquid
Recommend dilutions: ELISA (1:10000-1:50000)
Western Blot (1:1000-1:2500)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human
Application image: PAB10044-46-15-1.jpg
Application image note: Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate.
Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected.
Peptide competition (not shown) blocks staining of this band.
The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDyeâ„¢800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Applications: ELISA,IHC,WB-Ce
Shipping condition: Dry Ice
Publications: Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids.Harte PJ, Wu W, Carrasquillo MM, Matera AG.
Cytogenet Cell Genet. 1999;84(1-2):83-6.

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