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Data sheet of DNMT3L polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Tr |
More info about DNMT3L polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10043 |
| Product name: | DNMT3L polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of DNMT3L. |
| Gene id: | 29947 |
| Gene name: | DNMT3L |
| Gene alias: | MGC1090 |
| Gene description: | DNA (cytosine-5-)-methyltransferase 3-like |
| Immunogen: | A synthetic peptide corresponding to amino acids 152-164 of human DNMT3L. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000-1:3000) Western Blot (1:1500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Immunoblotting of DNMT3L polyclonal antibody (Cat # PAB10043) was usedat a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2-OS cells (10cm dish) was accomplished using 3 ug of GAL4-DNMT3L. Protein extraction using IPH at 150 mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 ug/mL in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 KDa. |
| Applications: | IP,ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Asynchronous replication timing of imprinted loci is independent of DNA methylation, but consistent with differential subnuclear localization.Gribnau J, Hochedlinger K, Hata K, Li E, Jaenisch R. Genes Dev. 2003 Mar 15;17(6):759-73. |