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Data sheet of BACH1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about BACH1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10027 |
| Product name: | BACH1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant BACH1. |
| Gene id: | 571 |
| Gene name: | BACH1 |
| Gene alias: | - |
| Gene description: | BTB and CNC homology 1, basic leucine zipper transcription factor 1 |
| Immunogen: | Recombinant protein corresponding to amino acids 92-104 of human BACH1. |
| Protein accession: | Q9BX63;NP_114432 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:44000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Expect reactivity with isoform 1 and isoform 2 of BACH1. |
| Reactivity: | Chimpanzee,Human |
| Application image: |  |
| Application image note: | Western blot using BACH1 polyclonal antibody (Cat # PAB10027) shows detection of a band at~105 KDa (Lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer.Clapperton JA, Manke IA, Lowery DM, Ho T, Haire LF, Yaffe MB, Smerdon SJ. Nat Struct Mol Biol. 2004 Jun;11(6):512-8. Epub 2004 May 9. |