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Data sheet of ANAPC10 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about ANAPC10 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10019 |
| Product name: | ANAPC10 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ANAPC10. |
| Gene id: | 10393 |
| Gene name: | ANAPC10 |
| Gene alias: | APC10|DKFZp564L0562|DOC1 |
| Gene description: | anaphase promoting complex subunit 10 |
| Immunogen: | A synthetic peptide corresponding to N-terminus of human ANAPC10. |
| Protein accession: | NP_055700.1 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:80000) Western Blot (1:500-1:1500) Immunohistochemistry (1:250-1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunoblotting of ANAPC10 polyclonal antibody (Cat # PAB10019) was used at a 1:500 dilution to detect human ANAPC10 by western blot. Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1 : 2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human ANAPC10 is 21 KDa. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex.Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM. J Biol Chem. 1999 May 14;274(20):14500-7. |