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Data sheet of ANAPC1 (phospho S355) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about ANAPC1 (phospho S355) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10017 |
| Product name: | ANAPC1 (phospho S355) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ANAPC1. |
| Gene id: | 64682 |
| Gene name: | ANAPC1 |
| Gene alias: | APC1|MCPR|TSG24 |
| Gene description: | anaphase promoting complex subunit 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S355 of human ANAPC1. |
| Protein accession: | Q9H1A4;NP_073153 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human APC1 protein at the pS355 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Reactivity: | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using ANAPC1 (phospho S355) polyclonal antibody (Cat # PAB10017) shows detection of a band ~215 KDa corresponding to phosphorylated human ANAPC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronizecells at the M phase. Phosphorylated ANAPC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat drymilk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. ECL reagent was used for detection. Data contributed by Bing Li, UT South western. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex.Jorgensen PM, Graslund S, Betz R, Stahl S, Larsson C, Hoog C. Gene. 2001 Jan 10;262(1-2):51-9. |