Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Orangutan,Pig,Rat |
Host species | Rabbit |
Applications | ELISA,IHC-P,WB-Re |
Brand: | Abnova |
Reference: | PAB10016 |
Product name: | HMGN1 (phospho S20/S24) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of HMGN1. |
Gene id: | 3150 |
Gene name: | HMGN1 |
Gene alias: | FLJ27265|FLJ31471|HMG14|MGC104230|MGC117425 |
Gene description: | high-mobility group nucleosome binding domain 1 |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S20/S24 of human HMGN1. |
Protein accession: | AAA52677;P05114 |
Form: | Liquid |
Recommend dilutions: | ELISA (1:5000-1:25000 Western Blot (1:500-1:2000) Immunohistochemistry (20 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Specificity: | Reactivity occurs against human HMGN pS20/pS24 proteins and This antibody is specific to the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA. |
Reactivity: | Bovine,Chicken,Clawed frog,Dog,Human,Mouse,Orangutan,Pig,Rat |
Application image: | ![]() |
Application image note: | Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. |
Applications: | ELISA,IHC-P,WB-Re |
Shipping condition: | Dry Ice |
Publications: | Human non-histone chromosomal protein HMG-17: identification, characterization, chromosome localization and RFLPs of a functional gene from the large multigene family.Landsman D, McBride OW, Bustin M. Nucleic Acids Res. 1989 Mar 25;17(6):2301-14. |