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Data sheet of FANCF polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about FANCF polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10011 |
| Product name: | FANCF polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of FANCF. |
| Gene id: | 2188 |
| Gene name: | FANCF |
| Gene alias: | FAF|MGC126856 |
| Gene description: | Fanconi anemia, complementation group F |
| Immunogen: | A synthetic peptide corresponding to internal region of human FANCF. |
| Protein accession: | NP_073562;Q9NPI8;Q52LM0 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:60000) Western Blot (1:1000-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Chimpanzee,Human |
| Application image: |  |
| Application image note: | Western blot using FANCF polyclonal antibody (Cat # PAB10011) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: implications for treatment and survival.Marsit CJ, Liu M, Nelson HH, Posner M, Suzuki M, Kelsey KT. Oncogene. 2004 Jan 29;23(4):1000-4. |