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Data sheet of EGFR polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Ce |
More info about EGFR polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10010 |
| Product name: | EGFR polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of EGFR. |
| Gene id: | 1956 |
| Gene name: | EGFR |
| Gene alias: | ERBB|ERBB1|HER1|PIG61|mENA |
| Gene description: | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Immunogen: | A synthetic peptide corresponding to amino acids 1189-1199 of human EGFR. |
| Protein accession: | P00533;NP_005219 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:8000-1:32000) Western Blot (1:500-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using EGFR polyclonal antibody (Cat # PAB10010) shows detection of aband at ~170 KDa (Lane 1) corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431whole cell lysates (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocell-ulose. After blocking the membrane was probedwith the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green). Molecular weight estimationwas made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: correlation with histopathologic features and receptors for estrogen, progesterone, and epidermal growth factor.Scambia G, Catozzi L, Panici PB, Ferrandina G, Coronetta F, Barozzi R, Baiocchi G, Uccelli L, Piffanelli A, Mancuso S. Am J Obstet Gynecol. 1993 Jan;168(1 Pt 1):71-8. |